Characterization of pro-apoptotic gene Bak using hammerhead ribozymes
نویسندگان
چکیده
منابع مشابه
Design, characterization and testing of tRNA3Lys-based hammerhead ribozymes.
A hammerhead ribozyme targeted against the HIV-1 env coding region was expressed as part of the anticodon loop of human tRNA3Lys without sacrificing tRNA stability or ribozyme catalytic activity. These tRNA-ribozymes were isolated from a library which was designed to contain linkers (sequences connecting the ribozyme to the anticodon loop) of random sequence and variable length. The ribozyme ta...
متن کاملCan hammerhead ribozymes be efficient tools to inactivate gene function?
In order to improve hammerhead ribozyme efficiency and specificity, we have analyzed, both in vitro and in vivo, the activity of a series of ribozyme/substrate combinations that have the same target sequence but differ in the length of the ribozyme/substrate duplex or in their structure, i.e., the total length of the RNA. In vitro, we have found that optimal kcat/Km (at 37 degrees C) is obtaine...
متن کاملExperimental cancer gene therapy by multiple anti-survivin hammerhead ribozymes.
To improve the efficacy of gene therapy for cancer, we designed four hammerhead ribozyme adenoviruses (R1 to R4) targeting the exposed regions of survivin mRNA. In addition to the in vitro characterization, which included a determination of the sequence specificity of cleavage by primer extension, assays for cell proliferation and for in vivo tumor growth were used to score for ribozyme efficie...
متن کاملbak, bax and nbk/bik pro-apoptotic gene alterations in iranian patients with ataxia telangiectasia
ataxia telangiectasia (at) is an autosomal recessive multi-system disorder, characterized by variable immunodeficiency, progressive neurodegeneration, occulocutaneous telangiectasia, and increased susceptibility to malignancies. this study was designed to study the role of pro-apoptotic bak, bax, nbk/bik genes in a group of patients with at to elucidate the possible role of these genes in progr...
متن کاملTargeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes.
Mortalin, also known as mot2/mthsp70/GRP75/PBP74, is a member of the heat-shock protein 70 family that is heat-uninducible. It is differentially distributed in cells that have normal and immortal phenotypes, has been localized to various subcellular sites, and has several binding partners and functions. Here, we describe the construction and use of mortalin-specific conventional and hybrid ribo...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2001
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/1.1.207